Identification of bovine herpesvirus-1 polypeptides involved in serum neutralization.
نویسندگان
چکیده
Bovine herpesvirus (infectious bovine rhinotracheitis virus)-infected cell antigens were solubilized with Nonidet P-40. The crude antigen extract was separated by reaction with bovine hyperimmune serum in line immunoelectrophoresis; individual immunoprecipitates were used to immunize rabbits. Rabbit sera possessing serum neutralizing activity were analyzed by reaction with crude antigen extract in immunoprecipitation sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE) and Western blot. Four virus-specified glycopeptides, with molecular weights of 69-75K, 77-81K, 82-92K and 108-115K, appeared to be involved in inducing serum neutralizing antibody.
منابع مشابه
Bovine herpesvirus 1: strain comparison of polypeptides and identification of a neutralization epitope on the 90-kilodalton hemagglutinin.
The intracellular and structural polypeptides of the Los Angeles and Cooper 1 reference strains of bovine herpesvirus 1, together with 12 other Canadian field isolates, were analyzed by polyacrylamide gel electrophoresis. Although a few minor differences were noted among some isolates in regard to intracellular viral protein content, analysis of partly purified virus showed strikingly similar p...
متن کاملIsolation of Bovine Herpesvirus-1 (BoHV-1) From Latently Infected/Carrier Cattle in Ahvaz
Bovine herpesvirus 1 (BoHV-1) is one of the most important infectious viruses of cattle which is considered as the causative agent of several clinical conditions in this species. Hence, we tried to isolate a local strain of the virus from carrier cattle in Ahvaz based on a strategy for reactivation of the latent infection in hosts having antibodies against the virus. Thus, 20 cattle were examin...
متن کاملA simple, specific, and highly sensitive blocking enzyme-linked immunosorbent assay for detection of antibodies to bovine herpesvirus 1.
By using a monoclonal antibody directed against an epitope located on glycoprotein B of bovine herpesvirus 1 (BHV1), a simple, convenient blocking enzyme-linked immunosorbent assay (ELISA) which combines a high sensitivity with a low false-positive rate has been developed. The test can be performed at low variance on undiluted bovine serum samples. The epitope on glycoprotein B appears to be co...
متن کاملSerological study of bovine viral diarrhea virus (BVDV) infection in water buffalo (Bubalusbubalis) in Ahvaz in the southwestern region of Iran
A serological survey was carried out to determine the prevalence rate of bovine viral diarrhea virus (BVDV) infections in water buffalo (Bubalusbubalis) in Ahvaz, which is the center of the Khouzestan province in Iran. For this purpose, blood samples were taken from 310 slaughtered buffaloes at the abattoir. Sera were tested via the serum neutralization test. Serum neutralization was performed ...
متن کاملRapid, sensitive, competitive serologic enzyme-linked immunosorbent assay for detecting serum antibodies to bovine herpesvirus type 1.
An enzyme-linked immunosorbent assay (ELISA) for the detection of cattle antibodies to bovine herpesvirus type 1 was developed on the basis of competition between serum antibody and a virus-neutralizing mouse monoclonal antibody. The assay showed improved sensitivity over the virus neutralization (VN) test and over an enhanced VN test in which incubation of antibody-virus mixtures was carried o...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Veterinary microbiology
دوره 11 3 شماره
صفحات -
تاریخ انتشار 1986